ABSTRACT The objective of this study was to evaluate how male age, semen extender, cooling (equilibration) time and freezing method influence the quality of Brahman bulls’ sperm after cryopreservation, as measured by Computer-Assisted Sperm Analysis (CASA) parameters. Six Brahman bulls, with an average age of 48.6±11.5 months, were used. Two ejaculates were collected for each animal on different days, with an interval of approximately one week between collections. Semen was diluted with three extenders: Andromed®, BioXcell®, and OptiXcell®. The samples […]