The objective of this study was to analyze the effect of the addition of alanine, glycine and glutamine on frozen seminal extender of Mangalarga marchador breed stallions. Five stallions were used from the Lugavi stables. Three ejaculates from each stallion were divided into six treatments: without the addition of amino acids, addition of 40 mM alanine, addition of 40 mM glycine, addition of 60 mM glutamine, addition of 7 mM glycine, alanine 7 mM + 20 mM glutamine and addition […]

Success rates in equine sperm cryopreservation are lower than in other domestic species. The objective of this study was to verify the effect of adding 0.1 IU/mL, 1 IU/mL and 10 IU/mL insulin to conventional frozen extender using computerized analysis of sperm motility (CASA), plasma membrane functionality by hypo-osmotic shock and acrossomal membrane integrity through the FITC/PSA assay. No difference was observed in treatment with 0.1 and 1 IU/mL after thawing in the analysis of motility and kinematic sperm parameters. […]

Semen samples of five specimens of Prochilodus lineatus (Valencienes, 1847) were used to test the toxic and cryoprotectants effects of six solutions based on BTS (Beltsville Thawing Solution®) 4.5%: A – BTS 4.5% + Methanol 10%; B – BTS 4.5% + DMSO 10%; C – BTS 4.5% + KCl 0.072% + Methanol 10%; D – BTS 4.5% + KCl 0.072% + DMSO 10%; E – BTS 4.5% + KI 0.036% + Methanol 10% and F – BTS 4.5% + KI […]

The freezing ability of semen from Alpine and Saanen goats submitted to an artificial photoperiod exposure were evaluated by the thermo-resistance (TTR) and hypoosmotic (HOST) tests and integrity of the acrosome using eight male goats, four of each breed and two age groups (young and adults). The quality of semen during the freezing process was superior for the young animals of both breeds. Spermatic motility immediately after thawing showed a positive effect on TTR; animals with higher motility after collection […]