R. Bras. Zootec.01/Feb/2010;39(2):273-8.

Adding insulin to frozen seminal extender from Mangalarga Marchador stallions

Bruno Fagundes, Maurício Fraga van Tilburg, José Frederico Straggiotti Silva, Aldo Shimoya, Marcus Antonio Pessanha Barreto, Vinicius Motta Ferreira

DOI: 10.1590/S1516-35982010000200007

Success rates in equine sperm cryopreservation are lower than in other domestic species. The objective of this study was to verify the effect of adding 0.1 IU/mL, 1 IU/mL and 10 IU/mL insulin to conventional frozen extender using computerized analysis of sperm motility (CASA), plasma membrane functionality by hypo-osmotic shock and acrossomal membrane integrity through the FITC/PSA assay. No difference was observed in treatment with 0.1 and 1 IU/mL after thawing in the analysis of motility and kinematic sperm parameters. However, when 10 IU/mL insulin was used there was a reduction in these parameters.

Adding insulin to frozen seminal extender from Mangalarga Marchador stallions

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