Two hundred fifty-two Hy-Line W36 laying hens were allotted in a randomized block design with 6 treatments, 7 replicates and 6 hens per experimental unit in order to determine the ideal digestible isoleucine: digestible lysine (Ile:Lys) ratio for laying hens aged 24-40 weeks. Experimental diets contained 5.92, 6.33, 6.73, 7.14, 7.54 and 7.95 g/kg of digestible isoleucine, resulting in different Ile:Lys ratios (0.73:1, 0.78:1, 0.83:1, 0.88:1, 0.93:1 and 0.98:1). Experimental diets were isonitrogenous, with different dietary contents of glutamic acid, […]