R. Bras. Zootec.19/Dec/2025;54:e20240190.
In vitro rumen fermentation of by-product from the production of edible mushroom Pleurotus ostreatus enriched or not with selenium
Maiane de Paula Alves
, Juliana Soares da Silva
, Adailton Camêlo Costa
, Diene France de Souza
, Robert Teixeira da Paixão
, Cláudia Batista Sampaio
, Hilário Cuquetto Mantovani
, Maria Catarina Megumi Kasuya
ABSTRACT
The objective of this study was to evaluate in vitro effects on ruminal fermentation of by-product from the production of edible mushroom Pleurotus ostreatus. The following by-products were tested: (I) non-colonized sugarcane bagasse (control); (II) sugarcane bagasse from non-enriched mushrooms; and (III) sugarcane bagasse from selenium-enriched mushrooms. A semi-automatic in vitro system was used. The pH and neutral detergent fiber (NDF) residue were determined at 0, 3, 6, 9, 12, 18, 24, 48, 72, and 96 h of incubation. The ruminal ammonia nitrogen (NH3-N) concentrations were measured at 0, 6, 12, 24 and 48 h incubation times and the short-chain fatty acids (SCFA) concentrations after 24 h incubation. The pH and NH3-N were not affected (P>0.05) by the treatments or the treatment × time interaction. However, an effect of incubation time was observed (P<0.05). The production of SCFA was not affected (P>0.05). Non-colonized sugarcane bagasse presented a higher value of the potentially degradable fraction (P<0.05) and a lower value of the undegradable fraction when compared to the other treatments (P<0.05). The results indicate that the by-products do not change the fermentative parameters.
Keywords: agro-industrial waste; fiber; ruminant
