The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
Cryopreservation of the semen of Nile tilapia Oreochromisniloticus, var. Chitralada: cryoprotectants, spermatozoa activating solutions and cryogenic refrigerator
Experiments were conducted concerning cryoprotectants, extenders, and post thawing activating solutions for the semen of Nile tilapia Oreochromisniloticus, var. Chitralada. The extender solutions contained DMSO or methanol in various concentrations. The semen was previously diluted in the extender, drawn into 0.5 mL straws, frozen in the cryogenic shipper and then stored in liquid nitrogen. Motility of the thawed sperm was evaluated with NaHCO3 119mM, NaCl 25 mM and distilled water. Motility rate of thawed sperm, cryopreserved with methanol (final concentration = 7.5-12%) and activated with NaHCO3 119mM, was 31-46%. When DMSO replaced methanol, the motility of frozen/thawed sperm activated, also with NaHCO3 119mM, was 20-35%. Semen:extender dilutions of 1:1 to 1:4 did not show significant effects on the post-thawing sperm motility rate.
Cryopreservation of the semen of Nile tilapia Oreochromisniloticus, var. Chitralada: cryoprotectants, spermatozoa activating solutions and cryogenic refrigerator
Godinho,Hugo Pereira and Amorim,Vanessa Márcia da Cunha and Peixoto,Marco Túlio Diniz. Cryopreservation of the semen of Nile tilapia Oreochromisniloticus, var. Chitralada: cryoprotectants, spermatozoa activating solutions and cryogenic refrigerator. R. Bras. Zootec. [online]. 2003, vol.32, 6 suppl.1, [cited 2026-04-23], pp.1537-1543. Available from: <https://rbz.org.br/article/cryopreservation-of-the-semen-of-nile-tilapia-oreochromis-niloticus-var-chitralada-cryoprotectants-spermatozoa-activating-solutions-and-cryogenic-refrigerator/>. ISSN 1516-3598. https://doi.org/10.1590/S1516-35982003000700001.
Godinho,Hugo Pereira; Amorim,Vanessa Márcia da Cunha; Peixoto,Marco Túlio Diniz. Cryopreservation of the semen of Nile tilapia Oreochromisniloticus, var. Chitralada: cryoprotectants, spermatozoa activating solutions and cryogenic refrigerator. R. Bras. Zootec., v.32, 6 suppl.1, p. 1537-1543. Dec. 2003. Available from: <https://rbz.org.br/article/cryopreservation-of-the-semen-of-nile-tilapia-oreochromis-niloticus-var-chitralada-cryoprotectants-spermatozoa-activating-solutions-and-cryogenic-refrigerator/>. acess on 23 Apr. 2026. https://doi.org/10.1590/S1516-35982003000700001.
Godinho,Hugo Pereira, & Amorim,Vanessa Márcia da Cunha, & Peixoto,Marco Túlio Diniz. (2003). Cryopreservation of the semen of Nile tilapia Oreochromisniloticus, var. Chitralada: cryoprotectants, spermatozoa activating solutions and cryogenic refrigerator. R. Bras. Zootec., 32(6 suppl.1) , 1537-1543. https://doi.org/10.1590/S1516-35982003000700001.
GodinhoHugo Pereira, AmorimVanessa Márcia da Cunha, PeixotoMarco Túlio Diniz. Cryopreservation of the semen of Nile tilapia Oreochromisniloticus, var. Chitralada: cryoprotectants, spermatozoa activating solutions and cryogenic refrigerator. R. Bras. Zootec. [Internet]. 2003 Dec [cited 2026 Apr 23]; 32(6 suppl.1): 1537-1543. Available from: https://rbz.org.br/article/cryopreservation-of-the-semen-of-nile-tilapia-oreochromis-niloticus-var-chitralada-cryoprotectants-spermatozoa-activating-solutions-and-cryogenic-refrigerator/. https://doi.org/10.1590/S1516-35982003000700001.
Figure 6
Differential abundance for the species Faecalibacterium prausnitzii (A) and Megamonas funiformis (B).
