R. Bras. Zootec.01/Dec/2011;40(12):2721-4.

Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay

Luiz Waldemar de Oliveira Souza, André Furugen Cesar Andrade, Eneiva Carla Carvalho Celeghini, João Alberto Negrão, Rubens Paes de Arruda

DOI: 10.1590/S1516-35982011001200015

The objectives of this study were to validate a non-extractive RIA for seminal testosterone and quantify the hormone using a solid-phase commercial kit, and study the correlation between testosterone in seminal plasma and sperm characteristics. Parallelism showed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R2 = 0.985), indicating that the non-extractive method presented is indicated particularly for assessment of testosterone when establishing comparisons between samples. Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlation was only found between the seminal concentrations of testosterone and pH of the semen.

Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay

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