In this work, a 19KDa, male specific protein, from bovine spermatozoa, was purified using gel filtration chromatography. A monoclonal antibody (Mab) developed (C11F) against this protein discriminated two populations of spermatozoa, as revealed by immunofluorescence in microscopy and flow citometry. The epitope recognized by Mab C11F was present in 49.7% of spermatozoa population. When added to fresh bovine semen, the Mab C11F reduced the motility and promoted agglutination of the spermatozoa.

The objective of this work was to study the effects of age of dam (AOD), age of calf (AOC) and Julian date of birth (DOB) on the average daily gain (ADG) from birth to weaning period of Nellore breed animals. The data were analyzed by restricted maximum likelihood, using the animal model. The model included the direct effects of contemporary group (CG), AOD, AOC and DOB, and the direct effects (animal), maternal, permanent environmental and residue. The covariates were defined […]

The objective of this experiment was to evaluate the fermentative quality of sorghum silage of small, medium and high size with different stem+leafs/head proportions (100:00, 80:20, 60:40, 40:60, 20:80, 00:100), by means of lab analysis. The whole plant was used as control. The hybrids were cultivated and ensiled at 104 days of age in PVC lab silos measuring 10-cm of diameter by 40-cm of length (three replications per treatment). Silos were opened 56 days after the ensilage. The crude protein […]

The objective of this experiment was to study the effects of population density on the productive performance of growing rabbits. One hundred and eight New Zealand White rabbits of both sexes, weaned at the 28 days of age and housed at the 35 days of age for an experimental period of 6 weeks, in galvanized wire cages with the dimensions of .6 x .6 x .375 m, equipped with automatic feeder and drinking nipple installed in partially shut housing. The […]

The objective of this research was to determine the correlation among hormone levels at 2- and 6-month of age and diameter and histology of the testis in Angus x Charolais young bulls at 6 months (n= 15). At two months, two basal blood samples were collected at 1-h intervals. After the last collection, the animals received 5 mg of GnRH and 1.5 and 3.0 h later, new samples were collected. At 6 months, the young bulls were treated with 1 […]

This work was to evaluate the effect of different forage levels in the diet on the intake and apparent digestibility of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), total carbohydrate (CHO), gross energy (GE) and total digestible nutrients (TDN). Thirty five pure Holstein bull calves, 60 days old with initial average live weight (LW) of 78 kg, were allotted in a completely randomized experimental design with nine replications per diet, except […]

The objective of this experiment was to determine the digestible lysine requirement for male broilers from 1 to 21 days. Six hundred male broiler chicks, Hubbard, with an average weight of 48 g were allotted to randomized blocks experimental design with five treatments, six replicates and 20 chicks per experimental unit. The treatments corresponded to the basal diet, with 20.85% of crude protein and 3000 kcal of ME/kg, attending the requirements of protein, energy, calcium, phosphorus and amino acids, except […]

The objective of this work was to evaluate the effects of different forage levels in the diet on the net and dietary calcium (Ca), phosphorus (P), magnesium (Mg), potassium (K) and sodium (Na) requirements. Forty-four pure Holstein bulls calves, 60 days old with initial average live weight (LW) of 78 kg were used. Eight reference animals were slaughtered at the beginning of the experiment, to estimate the initial empty body weight (EBW) and initial body concentrations of Ca, P, Mg, […]