The aim of this work was to analyze the genetic diversity of 14 Lotus corniculatus L. materials through microsatellite markers. Four cultivars and one population of L. corniculatus and their respective genotypes selected were analyzed aiming aluminium tolerance and sensibility. The 17 markers used detected a total of 36 alleles, with an average of 2.25 alleles per locus. The result of the similarity analyses showed the formation of three groups: one enclosing germplasm and selected genotypes for aluminium sensibility, while […]

The objective of this study was to evaluate the effects of supplementation with different sources of energy and nitrogenous compounds on the in vitro growth and production of bacteriocin of lactic acid bacteria. Incubations were performed by using ruminal fluid from a rumen-fistulated Holstein-Zebu steer. The animal was kept on a Brachiaria decumbens pasture receiving 200 g/day of supplemental crude protein. Substrates and inoculum were placed in glass flasks considering eight treatments: cellulose, cellulose and casein, cellulose and soy peptone, […]

The objective of this experiment was estimate sperm concentration of “dourado” (Salminus brasiliensis), “curimba” (Prochilodus lineatus), “jundiá” (Rhamdia quelen), “cascudo-preto” (Rhinelepis aspera) and Nile tilapia (Oreochromis niloticus) by the method of spermatocrit. It was used 19, 58, 51, 43 and 85 brood fish of “dourado”, “curimba”, “jundia”, “cascudo-preto” and Nile tilapia, respectively. Except for the Nile tilapia, the fishes were subjected to the hormonal induction process and then submitted to the semen collection. The measurement techniques of the sperm concentration […]

It was evaluated the effects of poultry litter treatment on moisture content, pH, density and volatilized ammonia for five consecutive flocks of broiler chicken breeding. It was used 640 birds per flock as a complete randomized design with eight treatments and four replicates. The treatments were the following: 1 non-treated litter; 2 litter submitted to in-house composting; 3 litter treated with aluminum sulfate; 4 litter submitted to gypsum; 5 litter treated with quicklime; 6 litter treated with dolomitic limestone; 7 […]

The objective of this study was to evaluate forage accumulation and morphogenetic and structural characteristics of marandu palisadegrass subjected to combinations of cutting heights and cutting intervals. The experiment was carried out from August 2003 to April 2004. Treatments corresponded to combinations between two cutting heights (15 and 30 cm) and three cutting intervals (time corresponding to the appearance of three, four and five new leaves per tiller), and they were allocated to experimental units according to a 2 × […]

The effects of skin temperature (TS) on the rate of heat loss by cutaneous evaporation (ES) in Holstein cows chronically exposed to sun, considering hair coat colour were studied. Sixteen purebred cows were measured for ES and TS at 01:00 p.m. after 6 hours of exposure to sun, on three body regions (flank, neck and gluteus) and considering dark and white spots separately. Sweating rate (S) and ES were measured by means of a ventilated capsule. Black skin areas presented […]

The objective of this study was to evaluate the effects of inclusion of by-product of fresh fruit (pineapple, guava, papaya, mango and passion fruit) used in the diet for bovines partly replacing corn silage on intake and total nutrient digestibility, on the production of microbial protein and the nitrogen balance and on prediction of digestible fractions and total digestible nutrients (TDN) of these by-products. All animals received corn silage and the urea/ammonium sulfate (9:1) mixture so diets would remain isonitrogenous. […]

This experiment was conducted to evaluate the effect of levels of calcium (Ca) and particle size of limestone (LS) for laying hens. It was used 216 Dekalb White laying hens at 25 to 49 weeks of age in a completely randomized design in a 3 × 2 factorial arrangement, composed of three levels of calcium (3.92, 4.02 and 4.12%) and two particle sizes of limestone (thin – 0.60 mm; and thick – 1.00 mm), resulting in 6 treatments with six […]

The objective of this work was to evaluate the effect of the levels of lactic and propionic acids on in vitro fermentation of ruminal microorganisms. In experiment 1, the levels, in a total of 12 were the following: addition of 0 (control 1), 50, 100, 150, 200 and 250 mM of lactic acid and 0 (control 2), 50, 100, 150, 200 and 250 mM of propionic acid, respectively, in incubation flasks, which contained ruminal inoculum, glucose and synthetic culture medium, […]

The objective of this study was to evaluate the different contents of neutral detergent fiber from forage at advanced maturity on intake, digestibility of the dry matter and of nutrients, nitrogen balance, milk production, efficiency metabolizable energy use and the ingestive behavior of lactating goats. Five dairy goats were distributed in 5 × 5 Latin square by using increasing contents of neutral detergent fiber from forage as independent variable. Concentrations of neutral detergent fiber from forage used were 20, 28, […]