The objective of this work was to select strains of Lactobacillus acidophilus to be used as a probiotic in calves.Strainsof Lactobacillus acidophilus (12) were isolated from calves feces and selected according to their acidifying capacities, resistance to hydrochloridric acid, lysozyme, phenol, biliary salt, antibiotics used in the calves productions, capacities of growing in ruminal liquid, and their capacities to inhibit a strain of enteropathogenic Escherichia coli in vitro. The isolates were classed as slower in lactic acid production. In test […]

Chemical composition and the potential and effective degradabilities of dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) of passion fruit residue (shelves) were studied in the varieties yellow (Passiflora edulis f. Flavicarpa Degener), purple (Passiflora edulis Sims) and sweet (Passiflora alata) and in the mixture of yellow and purple varieties. DM intake, ruminal concentrations of N-ammonium, volatiles fatty acids (VFA) and pH, and rate of liquid and solid rumen digesta flows were also studied. The residues (shelves) […]

This work was carried out to isolate, characterize and identify Lactobacillusacidophilus strain from calves feces with 1- 3 days of age, collected directly from the rectum. The following tests for the characterization of the isolates were done: cellular morphology, gran test, catalase, gas production from glucose, growth at 15 and 45ºC, Litmus milk reduction, growth in culture medium with 4 and 8% sodium chloride, arginine hydrolysis, nitrate reduction and fermentation of several “carbohydrates”. From a total of 526 initial isolates, […]

The objective of this study was to evaluate the intake, apparent digestibility coefficient (ADC), nitrogen balance, pH and nitrogen ammonia concentration (N-NH3) in the ruminal fluid of sheep. The animals were fed diets composed by concentrates with different ruminal degradability of the fractions starch and protein from the used sources: starch with high (triticale) and low (corn) ruminal degradability combined with nitrogen (N) sources of high (canola meal plus urea) and low (cottonseed meal plus meat and bone meal) ruminal […]

The ruminal dry matter (DM) and crude protein (CP) degradability of some feeds were evaluated by the in situ technique, using three Holstein cows. The concentrate feeds were ground through a 2 mm screen and the forage and the cassava root, in 5 mm screen, and incubated in nylon bag (53µ). The effective degradability (ED) of DM (5%/h) for corn silage, sorghum silage, corn, sorghum, wheat bran, triticale, cassava scraping, citrus pulp, soybean meal, cottonseed meal and meat and bone […]

This work was carried out to evaluate the physical characteristics of the digesta throughout the gastrointestinal tract and to exam the conception of critical particle size that escape from the reticulum-rumen (RR), in the dairy bull calves fed diets with different concentrate levels. Thirty two dairy bull calves, one half straightbred Holstein and half crossbreed Holstein-Zebu, slaughtered with average six months of age and 190 kg LW, after fasting for 16 hours. The diets fed libitum were constituted of coast-cross […]

The experiment was conducted to evaluate the dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) degradation kinetics and different models to estimate the passage rate in steers fed diets with 15.0% of broiler litter associated to two levels of a supplement based on bovine lyophilized ruminal microbiota (0 and 10 g/animal•day). Four steers with ruminal and abomasal fistulae were allotted to a completely randomized experiment. The passage rate was determined using chromium complexed to the cellular wall […]

The performance and digestibility of nutrients of lambs fed concentrates formulated with soybean meal (SM) and whole canola grain (WC), cracked canola grain (CC) or pelleted canola (PC) and oat hay, fed in a 30:70 (forage to concentrate ratio, %DM) were evaluated. Twenty-eight male lambs with initial age from 60 to 90 days and 17 kg LW were allotted to a completely randomized design. The intakes (g/d) of DM, CP, ADF, NDF and GE (Mcal/d), the average daily gain and […]

The starch solubility in bicarbonate-phosphate buffer (SSB) and the ruminal degradability of starch from some feed by the in situ technique, using three Holstein cows, were evaluated in this study. The feeds were ground through a 2-mm screen for the concentrate and a 5-mm screen for forage and incubated using nylon bag (53mm). The starch concentration in corn, sorghum, cassava scraping, wheat bran, triticale, corn silage, sorghum silage, soybean meal, cottonseed meal and citrus pulp were: 79.3, 83.4, 91.4, 49.1, […]

The milk production, the dry matter intake (DMI) and the NDF passage rate in the gastrointestinal tract in four seasons (July, October, January and March), of crossbred cows, under grazing of elephant grass, submitted to two levels of nitrogen (300 and 700 kg N/ha•year) were estimated. The rotation grazing with three days of occupation and 30 days of resting, using 36 crossbred Holstein Zebu milking cows, in a stocking rate of 6 cows/ha, was used. The estimates of the intake […]