The objective of this trial was to study the effects of increasing dietary levels of crude protein (CP) on microbial protein synthesis of lactating dairy goats. The microbial markers purines bases and purine derivatives (PD), measured in spot urine samples or in urine samples from total collection, were used to estimate microbial protein synthesis. Sixteen lactating goats (four fitted with ruminal cannula) were randomly assigned to four replicated 4 x 4 Latin squares and were fed the following dietary CP […]

Four ruminally cannulated Holstein steers averaging 259 kg of body weight were assigned to a 4×4 Latin square to study the effects of replacing elephantgrass silage with cottonseed hulls on serum urea nitrogen (SUN), ruminal metabolism, urinary excretion of nitrogenous compounds, and microbial protein synthesis measured by omasal purine bases or by urinary excretion of purine derivatives. Treatments (60% of forage) contained on DM basis: 0, 10, 20 or 30% of cottonseed hulls that partially replaced elephantgrass silage in the […]

Twelve Holstein cows were allotted to four 4 x 4 Latin square balanced in accordance with the lactation period, with the objective of evaluating the protein production, using the total purine derivatives (PD) excretion, obtained from spot urine collection, the plasma and milk urea and nitrogen(N)-urea, pH and ruminal ammonia concentration. The four experimental diets were formulated to contain 70% of corn silage and 30% of concentrate. Increasing levels of wheat middlings in the concentrate (0, 33, 67 and 100%) […]

Twelve purebred and crossbred Holstein cows were allotted to three 4 x 4 Latin Squares to evaluate the nitrogen (N) balance, microbial protein production, plasma and milk urea concentration and different urine collections times. The experimental diets were based on corn silage (CS) (AG 1051) with forage:concentrate ratio (F:C) of 60:40, in dry matter (DM) basis, or in sugar cane basis (SC) (RB 855536) with F:C of 60:40 with .35 or 1% of urea+ammonium sulfate (AS) mixture or FC of […]

This research was carried out, using 15 dairy cows (Holstein x Gyr) full fed isoproteic diets with 60% silage and 40% concentrate in the dry matter (DM) basis and 0, 0.7, 1.4, and 2.1% urea correspondent to 2.08, 4.01, 5.76, and 8.07% of crude protein levels in the form of non protein nitrogen compounds (NNP), with the objectives to a non invasive methodology, to estimate the microbial protein production, using the total excretion of purine derivatives (PD); to evaluate the […]

The objectives of the present work were to estimate the microbial protein production using the total purine derivatives (PD) excretion, to compare the PD and the urea excretions, obtained from spot urine collection with that observed in the 24 hours collection and to evaluate the plasma and milk N-urea and urea urinary excretion. Sixteen Holstein lactating cows were assigned to four 4X4 Latin Square with 3-wk periods in accordance with the lactation period. The four experimental isoproteic diets were formulated […]