ABSTRACT The objective of this study was to investigate the effects of recombinant adiponectin on chicken liver cells. The full-length chicken adiponectin gene was amplified by PCR and cloned into the vector pET-32a, followed by the transformation of the vector into Escherichia coli BL21. SDS-PAGE was used to detect and analyze the purity of the expressed recombinant protein. Induction was performed with 1 mM IPTG at 30 °C for 3 h, and the recombinant thioredoxin–adiponectin fusion protein was purified using […]

Four trials were conducted to determine the requirements of available phosphorus (aP) of female broiler chicks from 1 to 10 (exp.1), 11 to 21 (exp.2), 22 to 33 (exp.3), and from 34 to 46 (exp.4) days of age, keeping the calcium:available phosphorus ratio equal to 2:1. In each trial, 480 Cobb broilers were randomly allotted in a completely randomized design with 6 treatments, 8 replicates and 10 birds per pen. The levels of aP ranged from 2.0 to 5.5 g/kg […]

Long-term selection experiments provide a valuable resource for understanding the genetic changes that take place in animal improvement programs. The Virginia chicken lines have for more than 50 years been subjected to single-trait, bi-directional, divergent selection for high and low juvenile body weight. It is one of the most well studied experimental populations in agricultural animals and has played an instrumental role in the understanding of the genetic basis of complex traits. In this paper, we summarize the findings from […]

The objective of this study was to evaluate the effects of vitamin and micromineral levels adjustment in the test diet on the total collection method for determination of apparent metabolizable energy (EMA) and apparent metabolizable energy corrected by nitrogen retention (EMAn) of soybean meal. Two treatments were compared: the test diet without or with adjustments in the amounts of choline chloride and vitamin-micromineral premix, when 40% of the reference diet was replaced with soybean meal. The traditional total feces collection […]

Four hundred and eighty broilers were assigned to a completely randomized design with a 6×2 arrangement (six levels of manganese and 2 sexes – male and female) and four replicates to determine the manganese requirements in the growing (22 to 42 days) and finishing (43 to 54 days) phases. Six and four birds per experimental unit, respectively, were used in the first and second trials. In both trials, the treatments consisted of a basal diet deficient in manganese (6.5 ppm) […]