The objective was to evaluate the effects of daily intake of four levels of phosphorus (8, 12, 15 and 18 g) on the metabolism of macro minerals (Ca, P, Mg, Na, K and S), including the intake, concentration in rumen, ruminal fluid passage rate, excretion in feces and apparent availability. Four rumen-fistulated buffaloes were allotted to a 4 × 4 Latin Square, with total diet composed by sugar cane as forage (85%) and concentrate formulated for each different phosphorus levels. […]