The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
In this work, a 19KDa, male specific protein, from bovine spermatozoa, was purified using gel filtration chromatography. A monoclonal antibody (Mab) developed (C11F) against this protein discriminated two populations of spermatozoa, as revealed by immunofluorescence in microscopy and flow citometry. The epitope recognized by Mab C11F was present in 49.7% of spermatozoa population. When added to fresh bovine semen, the Mab C11F reduced the motility and promoted agglutination of the spermatozoa.
Monoclonal antibody against male-specific protein of 19 KDa from bovine spermatozoa: a successful methodology for imunosexing
Souza,Clóvis José Pascarelli and Matta,Marcos Fernandes Resende da and Cruz,Gilson Mendes and Alves,Elias Walter and Kanashiro,Milton Massaricho and Silva,José Frederico Straggiotti. Monoclonal antibody against male-specific protein of 19 KDa from bovine spermatozoa: a successful methodology for imunosexing. R. Bras. Zootec. [online]. 1999, vol.28, 1, [cited 2026-03-08], pp.74-78. Available from: <https://rbz.org.br/article/monoclonal-antibody-against-male-specific-protein-of-19-kda-from-bovine-spermatozoa-a-successful-methodology-for-imunosexing/>. ISSN 1516-3598. http://doi.org/10.1590/S1516-35981999000100010.
Souza,Clóvis José Pascarelli; Matta,Marcos Fernandes Resende da; Cruz,Gilson Mendes; Alves,Elias Walter; Kanashiro,Milton Massaricho; Silva,José Frederico Straggiotti. Monoclonal antibody against male-specific protein of 19 KDa from bovine spermatozoa: a successful methodology for imunosexing. R. Bras. Zootec., v.28, 1, p. 74-78. Feb. 1999. Available from: <https://rbz.org.br/article/monoclonal-antibody-against-male-specific-protein-of-19-kda-from-bovine-spermatozoa-a-successful-methodology-for-imunosexing/>. acess on 08 Mar. 2026. http://doi.org/10.1590/S1516-35981999000100010.
Souza,Clóvis José Pascarelli, & Matta,Marcos Fernandes Resende da, & Cruz,Gilson Mendes, & Alves,Elias Walter, & Kanashiro,Milton Massaricho, & Silva,José Frederico Straggiotti. (1999). Monoclonal antibody against male-specific protein of 19 KDa from bovine spermatozoa: a successful methodology for imunosexing. R. Bras. Zootec., 28(1) , 74-78. http://doi.org/10.1590/S1516-35981999000100010.
SouzaClóvis José Pascarelli, MattaMarcos Fernandes Resende da, CruzGilson Mendes, AlvesElias Walter, KanashiroMilton Massaricho, SilvaJosé Frederico Straggiotti. Monoclonal antibody against male-specific protein of 19 KDa from bovine spermatozoa: a successful methodology for imunosexing. R. Bras. Zootec. [Internet]. 1999 Feb [cited 2026 Mar 08]; 28(1): 74-78. Available from: https://rbz.org.br/article/monoclonal-antibody-against-male-specific-protein-of-19-kda-from-bovine-spermatozoa-a-successful-methodology-for-imunosexing/. http://doi.org/10.1590/S1516-35981999000100010.
Figure 3
Ruminal disappearance of ash-free neutral detergent fiber (aNDFom) at 30 h of in situ incubation in corn husks (fresh and ensiled) treated with calcium oxide (CaO) and a mixture of corn heavy steep water and sugarcane molasses (HM).
