R. Bras. Zootec.01/Aug/2006;35(4):1336-41.
Genetic characterization of Brazilian native breeds of goats using 27 markers microsatellites
DOI: 10.1590/S1516-35982006000500012
This study aimed to evaluate 27 microsatellites for genetic characterization of Brazilian native goats using samples of hair collected from 332 animals of the Moxotó, Canindé, Serrana Azul, Marota, Repartida and Graúna breeds. Twenty-seven microsatellites were selected, amplified by polymerase chain reaction (PCR). The amplified products were submitted to electrophoreses in poliacrylamide gel using an ABI 377XL automatic sequencer. The number of alleles ranged between three (MAF209) and twenty-three (CSSM66). Averages of expected and observed heterozigosity were respectively 0,672 and 0,582. Except for the marker MAF209 (PIC: 0,042), all the analyzed microsatellites were informative. The polymorphism of all microsatellites indicate their hability in identifying genetic variability of Brazilian native breeds of goats.
Keywords: genetic conservation; genetic variability; goats; molecular markers