The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
One hundred and twenty estrus cycles of 105 Mangalarga Marchador breed mares were analyzed to study the effect of number of inseminations per cycle on the fertility of mares inseminated with cooled semen. The mares were randomly allocated in the following group: T1 – one AI/cycle; T2 – two AI/cycle; T3 – tree or more AI/cycle. The cycles were controlled by rectal palpation and teasing. The insemination were made at each 48 hours, from a 30-35 mm follicle diameter until ovulation, with diluted, cooled (20ºC) and transported equine semen. The conception rates in the first cycle were, respectively, to T1, T2 and T3, 47.83% (11/23), 70.00% (21/30), and 45.16% (14/31) (P>0,05), and after five cycles were 51.43% (18/35), 48.89% (22/45) and 47.50% (19/40) (P>0,05). The number of AI/cycle had no effect fertility in mares.
Effect of the Number of Insemination per Cycle on Fertility of Inseminated Mares with Diluted Equine Semen, Cooled to 20oC and Transported
Lima,Marcos Chalhoub Coelho and Filho,José Monteiro da Silva and Carvalho,Giovanni Ribeiro de and Palhares,Maristela Silveira and Valle,Guilherme Ribeiro and Oliveira,Henrique Nunes. Effect of the Number of Insemination per Cycle on Fertility of Inseminated Mares with Diluted Equine Semen, Cooled to 20oC and Transported. R. Bras. Zootec. [online]. 2000, vol.29, 6, [cited 2026-03-17], pp.1649-1653. Available from: <https://rbz.org.br/article/effect-of-the-number-of-insemination-per-cycle-on-fertility-of-inseminated-mares-with-diluted-equine-semen-cooled-to-20oc-and-transported/>. ISSN 1516-3598. http://doi.org/10.1590/S1516-35982000000600008.
Lima,Marcos Chalhoub Coelho; Filho,José Monteiro da Silva; Carvalho,Giovanni Ribeiro de; Palhares,Maristela Silveira; Valle,Guilherme Ribeiro; Oliveira,Henrique Nunes. Effect of the Number of Insemination per Cycle on Fertility of Inseminated Mares with Diluted Equine Semen, Cooled to 20oC and Transported. R. Bras. Zootec., v.29, 6, p. 1649-1653. Dec. 2000. Available from: <https://rbz.org.br/article/effect-of-the-number-of-insemination-per-cycle-on-fertility-of-inseminated-mares-with-diluted-equine-semen-cooled-to-20oc-and-transported/>. acess on 17 Mar. 2026. http://doi.org/10.1590/S1516-35982000000600008.
Lima,Marcos Chalhoub Coelho, & Filho,José Monteiro da Silva, & Carvalho,Giovanni Ribeiro de, & Palhares,Maristela Silveira, & Valle,Guilherme Ribeiro, & Oliveira,Henrique Nunes. (2000). Effect of the Number of Insemination per Cycle on Fertility of Inseminated Mares with Diluted Equine Semen, Cooled to 20oC and Transported. R. Bras. Zootec., 29(6) , 1649-1653. http://doi.org/10.1590/S1516-35982000000600008.
LimaMarcos Chalhoub Coelho, FilhoJosé Monteiro da Silva, CarvalhoGiovanni Ribeiro de, PalharesMaristela Silveira, ValleGuilherme Ribeiro, OliveiraHenrique Nunes. Effect of the Number of Insemination per Cycle on Fertility of Inseminated Mares with Diluted Equine Semen, Cooled to 20oC and Transported. R. Bras. Zootec. [Internet]. 2000 Dec [cited 2026 Mar 17]; 29(6): 1649-1653. Available from: https://rbz.org.br/article/effect-of-the-number-of-insemination-per-cycle-on-fertility-of-inseminated-mares-with-diluted-equine-semen-cooled-to-20oc-and-transported/. http://doi.org/10.1590/S1516-35982000000600008.
Figure 3
Ruminal disappearance of ash-free neutral detergent fiber (aNDFom) at 30 h of in situ incubation in corn husks (fresh and ensiled) treated with calcium oxide (CaO) and a mixture of corn heavy steep water and sugarcane molasses (HM).