The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The objective of this work was to evaluate the ruminal disappearance and nitrogen intestinal digestibility of 18 concentrate feedstuffs and four grasses, using an in vitro inhibitor with HCL-pepsin-pancreatin solutions and the three-step method. The disappearance of nitrogen compounds of feedstuffs was determined at 0 and 2 hours using 1.875 mg of N incubated on each three vessels. One of the vessels was submitted to intestinal digestion. On the second method, approximately 5 g of samples were incubated, during 16 hours, in the rumen of a steer fed a diet with 60:40 forage to concentrate ratio. After the incubation period, samples containing 15 mg of residual N were incubated in HCL-pepsin-pancreatin solutions during 25 hours. Data of intestinal digestibility of ruminal undegradable protein was not constant. The inhibitor method was more efficient to evaluate and predict kinetic parameters of ruminal degradation and the three-step method for intestinal digestibility of ruminal undegradable protein.
Application of Two in Vitro Methods for Evaluation of Ruminal Disappearance and Intestinal Digestibility of Feed Protein
Hernández,Fernando Iván Londoño and Filho,Sebastião de Campos Valadares and Lana,Rogério de Paula and Mancio,Antonio Bento and Paulino,Mario Fonseca and Cecon,Paulo Roberto and Valadares,Rilene Ferreira Diniz and Magalhães,Karla Alves and Rosa,Reis Sandro Luiz. Application of Two in Vitro Methods for Evaluation of Ruminal Disappearance and Intestinal Digestibility of Feed Protein. R. Bras. Zootec. [online]. 2002, vol.31, 1, [cited 2026-03-15], pp.256-260. Available from: <https://rbz.org.br/article/application-of-two-in-vitro-methods-for-evaluation-of-ruminal-disappearance-and-intestinal-digestibility-of-feed-protein/>. ISSN 1516-3598. http://doi.org/10.1590/S1516-35982002000100028.
Hernández,Fernando Iván Londoño; Filho,Sebastião de Campos Valadares; Lana,Rogério de Paula; Mancio,Antonio Bento; Paulino,Mario Fonseca; Cecon,Paulo Roberto; Valadares,Rilene Ferreira Diniz; Magalhães,Karla Alves; Rosa,Reis Sandro Luiz. Application of Two in Vitro Methods for Evaluation of Ruminal Disappearance and Intestinal Digestibility of Feed Protein. R. Bras. Zootec., v.31, 1, p. 256-260. Feb. 2002. Available from: <https://rbz.org.br/article/application-of-two-in-vitro-methods-for-evaluation-of-ruminal-disappearance-and-intestinal-digestibility-of-feed-protein/>. acess on 15 Mar. 2026. http://doi.org/10.1590/S1516-35982002000100028.
Hernández,Fernando Iván Londoño, & Filho,Sebastião de Campos Valadares, & Lana,Rogério de Paula, & Mancio,Antonio Bento, & Paulino,Mario Fonseca, & Cecon,Paulo Roberto, & Valadares,Rilene Ferreira Diniz, & Magalhães,Karla Alves, & Rosa,Reis Sandro Luiz. (2002). Application of Two in Vitro Methods for Evaluation of Ruminal Disappearance and Intestinal Digestibility of Feed Protein. R. Bras. Zootec., 31(1) , 256-260. http://doi.org/10.1590/S1516-35982002000100028.
HernándezFernando Iván Londoño, FilhoSebastião de Campos Valadares, LanaRogério de Paula, MancioAntonio Bento, PaulinoMario Fonseca, CeconPaulo Roberto, ValadaresRilene Ferreira Diniz, MagalhãesKarla Alves, RosaReis Sandro Luiz. Application of Two in Vitro Methods for Evaluation of Ruminal Disappearance and Intestinal Digestibility of Feed Protein. R. Bras. Zootec. [Internet]. 2002 Feb [cited 2026 Mar 15]; 31(1): 256-260. Available from: https://rbz.org.br/article/application-of-two-in-vitro-methods-for-evaluation-of-ruminal-disappearance-and-intestinal-digestibility-of-feed-protein/. http://doi.org/10.1590/S1516-35982002000100028.
Figure 3
Ruminal disappearance of ash-free neutral detergent fiber (aNDFom) at 30 h of in situ incubation in corn husks (fresh and ensiled) treated with calcium oxide (CaO) and a mixture of corn heavy steep water and sugarcane molasses (HM).
