ABSTRACT The objective of this study was to determine the efficiency of Ringer’s lactate solution (RL) and RL + 1% fetal bovine serum (FBS) and compare them with the efficiency of Dulbecco’s phosphate-buffered saline (D-PBS). Twenty-two Wagyu female cattle were subjected to superovulation and were randomly distributed to form three groups: group 1 – uterine flushing with RL (n = 8), group 2 – uterine flushing with RL + 1% FBS (n = 7), and group 3 – uterine flushing […]

The effects of feeding urea on embryo production, quality and developmental stages and follicular status at the beginning of a superovulatory treatment of Toggenburg does fed urea diet were investigated in this study. Eighteen females were randomly allocated to receive diets with: no urea (control, n=8), and 2.4% urea dietary dry matter (n=10) UDM. The embryo recovery was performed at day 7 or 8 of estrus cycle by transcervical technique and classified according to quality and developmental stage. The follicular […]

This work was carried out to study the effect of dry matter feed intake level (DMI/% BW), weight gain, production and viability of embryos in cross-breed heifers so does production and viability of embryonic structures in Nellore cows. During the first trial, 27 cross-breed (½ Nelore x ½ Simental) heifers were used divided in 3 treatments and 9 replications: 1.2%; 1.6% and 2.6% of DMI/day in relation to body weight, respectively. The heifers were synchronized and after nine days these […]

Seventeen Nelore heifers were submitted to a superovulation protocol, beginning on the ninth day of the estrous cycle. Eight heifers were superovulated with 250 IU (T1) and nine with 500 IU (T2) of FSH. The animals were monitored by ultrasonography during the superovulation period and on the embryo collection day. There were no differences on the recovery rate and quality of the structures collected among the animals of the two treatments. Animals treated with 250 (T1) and 500 IU (T2) […]

The objective of this experiment was to study the progesterone plasma concentrations in crossbred heifers, and the correlation with lipid metabolites concentrations. Twenty-six heifers were distributed into two treatments: T1 – treated with 500 mg sometribove zinc (recombinant bovine somatotropin) on day 3 of superovulated estrous cycle and T2 – control. The blood samples were collected during two estrous cycles: normal and superovulated one. The progesterone and metabolic lipids concentrations were determined by RIA and enzymatic method, respectively. The experimental […]