The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
The Revista Brasileira de Zootecnia (RBZ) is a publication dedicated to the broad field of Animal Science. We publish high-quality, original scientific research that spans across diverse areas within the discipline. The scope of RBZ encompasses a wide range of topics, including aquaculture, biometeorology and animal welfare, forage crops and grasslands, animal and forage plants breeding and genetics, animal reproduction, ruminant and non-ruminant nutrition, meat science and muscle biology, precision livestock, and animal production systems and agribusiness.
01/Feb/1999
Clóvis José Pascarelli Souza, Marcos Fernandes Resende da Matta, Gilson Mendes Cruz, Elias Walter Alves, Milton Massaricho Kanashiro, José Frederico Straggiotti Silva
DOI: 10.1590/S1516-35981999000100010
In this work, a 19KDa, male specific protein, from bovine spermatozoa, was purified using gel filtration chromatography. A monoclonal antibody (Mab) developed (C11F) against this protein discriminated two populations of spermatozoa, as revealed by immunofluorescence in microscopy and flow citometry. The epitope recognized by Mab C11F was present in 49.7% of spermatozoa population. When added to fresh bovine semen, the Mab C11F reduced the motility and promoted agglutination of the spermatozoa.
Keywords: bovines; immunosexing; male specific protein; monoclonal antibody; spermatozoa