The objective of this experiment was to study the in vitro effects of the ionophores on ruminal fermentation of amino acids. Rumen fluid of a steer fed an elephant-grass based diet was used in the incubations, with addition of casein hydrolysate, in three treatments (control-C; monensin-M; and lasalocid-L). Inocula were transferred into new tubes, daily and on the 11th day of incubation, tubes from each of the treatments were used to inoculate three new tubes (C, M, L), totalizing nine […]