With the objective to establish the nutritional requirement of lysine for light and semi-heavy laying hens, in the period from 34 to 50 weeks old, 360 laying hens (half Lohmann and half Lohmann Brown) were submitted to a basal ration containing 2.850 kcal ME/kg, 15.0% CP, supplemented with five levels (0.00, 0.05, 0.10, 0.15 and 0.20%) of L-lysine, to provide 0.584, 0.634, 0.684, 0.734, and 0.784% of digestible lysine in the rations. A 5 x 2 (lysine level and laying […]